GreenGate Cloning System
Introduction
The 56 plasmid GreenGate cloning kit can be used to create plant expression vectors containing several cassettes and generate multi-construct transgenic plants.
Depositor
MGLCC accession number
Kit #1000000036
RM700.00
Description
GreenGate is a simple and efficient cloning system for rapidly assembling plant transformation constructs . It is based on the Golden Gate method. Using the type IIS restriction endonuclease BsaI and T4 DNA ligase, ready-to-use plant transformation vectors are built from six types of pre-cloned insert modules and a destination vector in a one-pot reaction. The six module types are (1) plant promoter, (2) N-terminal tag, (3) coding sequence (i.e. the gene of interest), (4) C-terminal tag, (5) plant terminator and (6) plant resistance cassette.
| Accession Number | Name | Properties And Application | hf:categories |
|---|---|---|---|
| Kit #1000000036 | GreenGate Cloning System | kit | |
| 48856 | pGGA000 | (Empty Backbone) Empty entry vector for creating GreenGate promoter modules. | plasmid |
| 48813 | pGGA002 | Provides the AP3 promoter as GreenGate module. | plasmid |
| 48814 | pGGA003 | Provides the WUS promoter as GreenGate module. | plasmid |
| 48815 | pGGA004 | Provides the 35S promoter as GreenGate module. | plasmid |
| 48816 | pGGA006 | Provides the UBQ10 promoter as GreenGate module. | plasmid |
| 48817 | pGGA008 | Provides the alcA promoter as GreenGate module. | plasmid |
| 48818 | pGGA012 | Provides the RPS5A promoter as GreenGate module. | plasmid |
| 48857 | pGGB000 | (Empty Backbone) Empty entry vector for creating GreenGate N-terminal tag modules. | plasmid |
| 48819 | pGGB001 | Provides mCherry:linker as GreenGate N-terminal tag module. | plasmid |
| 48820 | pGGB002 | Provides the omega element as GreenGate N-terminal tag module. | plasmid |
| 48821 | pGGB003 | Provides a default dummy as GreenGate N-terminal tag module. | plasmid |
| 48822 | pGGB005 | Provides the SV40 nuclear localization signal as GreenGate N-terminal tag module. | plasmid |
| 48823 | pGGB006 | Provides an ER signal sequence as GreenGate N-terminal tag module. | plasmid |
| 48858 | pGGC000 | (Empty Backbone) Empty entry vector for creating GreenGate coding sequence modules. | plasmid |
| 48824 | pGGC001 | Provides WUS as GreenGate coding sequence module. | plasmid |
| 48825 | pGGC011 | Provides alcR as GreenGate coding sequence module. | plasmid |
| 48826 | pGGC012 | Provides GFP:NLS as GreenGate coding sequence module. | plasmid |
| 48827 | pGGC014 | Provides GFP as GreenGate coding sequence module. | plasmid |
| 48828 | pGGC015 | Provides mCherry as GreenGate coding sequence module. | plasmid |
| 48829 | pGGC024 | Provides BFP as GreenGate coding sequence module. | plasmid |
| 48830 | pGGC025 | Provides 3xGFP as GreenGate coding sequence module. | plasmid |
| 48831 | pGGC026 | Provides 3xmCherry as GreenGate coding sequence module. | plasmid |
| 48832 | pGGC051 | Provides GUS as GreenGate coding sequence module. | plasmid |
| 48859 | pGGD000 | (Empty Backbone) Empty entry vector for creating GreenGate C-terminal tag modules. | plasmid |
| 48833 | pGGD001 | Provides linker:GFP as GreenGate C-terminal tag module. | plasmid |
| 48834 | pGGD002 | Provides a default dummy as GreenGate C-terminal tag module. | plasmid |
| 48835 | pGGD003 | Provides linker:mCherry as GreenGate C-terminal tag module. | plasmid |
| 48853 | pGGD005 | Provides linker:BFP as GreenGate C-terminal tag module. | plasmid |
| 48836 | pGGD006 | Provides the SV40 nuclear localization signal as GreenGate C-terminal tag module. | plasmid |
| 48837 | pGGD007 | Provides linker:nuclear localization signal as GreenGate C-terminal tag module. | plasmid |
| 48838 | pGGD008 | Provides an ER retrieval signal as GreenGate C-terminal tag module. | plasmid |
| 48860 | pGGE000 | (Empty Backbone) Empty entry vector for creating GreenGate terminator modules. | plasmid |
| 48839 | pGGE001 | Provides the rbcS terminator as GreenGate module. | plasmid |
| 48840 | pGGE002 | Provides the WUS terminator as GreenGate module. | plasmid |
| 48854 | pGGE003 | Provides the At1g04880 terminator as GreenGate module. | plasmid |
| 48855 | pGGE005 | Provides the At1g76110 terminator as GreenGate module. | plasmid |
| 48841 | pGGE009 | Provides the UBQ10 terminator as GreenGate module. | plasmid |
| 48861 | pGGF000 | (Empty Backbone) Empty entry vector for creating GreenGate plant resistance cassette modules. | plasmid |
| 48842 | pGGF001 | Provides a BASTA resistance cassette as GreenGate module. | plasmid |
| 48843 | pGGF002 | Provides a BASTA resistance cassette as GreenGate module. | plasmid |
| 48844 | pGGF003 | Provides a D-alanine resistance cassette as GreenGate module. | plasmid |
| 48845 | pGGF004 | Provides a D-alanine resistance cassette as GreenGate module. | plasmid |
| 48846 | pGGF005 | Provides a hygromycin resistance cassette as GreenGate module. | plasmid |
| 48847 | pGGF007 | Provides a kanamycin resistance cassette as GreenGate module. | plasmid |
| 48848 | pGGF008 | Provides a BASTA resistance cassette as GreenGate module. | plasmid |
| 48849 | pGGF012 | Provides a sulfadiazine resistance cassette as GreenGate module. | plasmid |
| 48850 | pGGG001 | Provides an F-H adapter as GreenGate module. | plasmid |
| 48851 | pGGG002 | Provides an H-A adapter as GreenGate module. | plasmid |
| 48862 | pGGH000 | (Empty Backbone) Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag + terminator modules. | plasmid |
| 48863 | pGGI000 | (Empty Backbone) Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag modules. | plasmid |
| 48864 | pGGM000 | (Empty Backbone) Empty entry vector for creating GreenGate supermodules w/o plant resistance cassette. | plasmid |
| 48865 | pGGN000 | (Empty Backbone) Empty entry vector for creating GreenGate supermodules with plant resistance cassette. | plasmid |
| 48866 | pGGY001 | (Empty Backbone) Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border. | plasmid |
| 48867 | pGGY003 | (Empty Backbone) Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border. | plasmid |
| 48868 | pGGZ001 | (Empty Backbone) Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border. | plasmid |
| 48869 | pGGZ003 | (Empty Backbone) Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border. | plasmid |