pGGF002
Properties and application
Provides a BASTA resistance cassette as GreenGate module.
MGLCC accession number
48843
RM350.00
Purified plasmid
Product Information
| Vector backbone | pUC19 |
| Vector type | Golden Gate compatible cloning vector |
| Bacterial resistance | Ampicillin |
| Growth temperature | 37°C |
| Host range | Mach1 |
| Copy number | High Copy |
| Cloned gene | p35S::BastaR::t35S |
| Cloned gene (alt name) | Basta resistance cassette |
| Gene size, bp | 1628 |
| Mutation | internal BsaI recognition site in 35S promoter removed by substitution |
| Species | Synthetic |
| Comment | This plasmid is designed for Golden Gate cloning using the BsaI enzyme. Plasmid Features (listed as bp in full plasmid sequence): BsaI site #1 = 2241-2251bp BASTA resistance cassette with 35S promoter and terminator = 2252-3879bp BsaI site #2 = 3880-3890bp |
