pGGA000
Properties and application
(Empty Backbone) Empty entry vector for creating GreenGate promoter modules.
MGLCC accession number
48856
RM350.00
Purified plasmid
Product Information
| Vector backbone | pUC19 |
| Vector type | Golden Gate compatible cloning vector |
| Bacterial resistance | Chloramphenicol and Ampicillin |
| Growth temperature | 37°C |
| Host range | DB3.1 |
| Copy number | High Copy |
| Cloned gene | None |
| Cloned gene (alt name) | - |
| Gene size, bp | - |
| Mutation | - |
| Species | - |
| Comment | This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI. Plasmid Features (listed as bp in full plasmid sequence): BsaI site #1 = 2241-2251bp BsaI site #2 = 3315-3325bp |
