Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia

Work Hours
Monday to Friday: 830AM - 530PM



Purpose / Description: Provides the AP3 promoter as GreenGate module.
Reference: GreenGate – A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis.
Verification: None
Depositor(s): Jan Lohmann
Catalog ID: 48858

Product details

Bacteria Resistance: Chloramphenicol and Ampicillin
Vector backbone: pUC19
Vector type: Golden Gate compatible cloning vector
Backbone size without insert: 2686 bp
Copy number: High
Growth strain: DB3.1
Growth temperature: 37°C
Antibiotics Resistance: Unknown
Depositor comment: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5′ BsaI overhang can be exchanged using HindIII and BamHI. The 3′ BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.

Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Gene / Insert: None