Address
Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia

Work Hours
Monday to Friday: 830AM - 530PM

pGGC001

RM150.00

Purpose / Description: Provides the AP3 promoter as GreenGate module.
Reference: GreenGate – A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis.
Verification: None
Depositor(s): Jan Lohmann
Catalog ID: 48824

Product details

Bacteria Resistance: Ampicillin
Vector backbone: pUC19
Vector type: Golden Gate compatible cloning vector
Backbone size without insert: 2686 bp
Copy number: High
Growth strain: Mach1
Growth temperature: 37°C
Antibiotics Resistance: Unknown
Depositor comment: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.

Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
WUS coding sequence = 2252-3129bp
BsaI site #2 = 313-314bp
Gene / Insert: WUS (At2g1795 4.4 kb promoter)
Insert size: 878 bp
Species: A. thaliana (mustard weed)
Mutation: internal BsaI recognition site removed by silent substitution