pGGY003
Properties and application
(Empty Backbone) Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.
MGLCC accession number
48867
RM350.00
Purified plasmid
Product Information
| Vector backbone | pGreenII |
| Vector type | Golden Gate compatible plant transformation vector |
| Bacterial resistance | Chloramphenicol and Gentamycin |
| Growth temperature | 37°C |
| Host range | DB3.1 |
| Copy number | High Copy |
| Cloned gene | None |
| Cloned gene (alt name) | - |
| Gene size, bp | - |
| Mutation | - |
| Species | - |
| Comment | This plasmid is designed as destination vector for Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using KpnI and EcoRI. The 3' BsaI overhang can be exchanged using XhoI and XbaI. There is also a a NotI-PacI-SgsI-SfaAI-KpnI MCS in front of the A-overhang and an XbaI-PaeI MCS after the G-overhang. Plasmid Features (listed as bp in full plasmid sequence): BsaI site #1 = 602-612bp BsaI site #2 = 2040-2050bp |
