Address
Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia
Work Hours
Monday to Friday: 830AM - 530PM
pGGY001
RM150.00
Purpose / Description: Provides the AP3 promoter as GreenGate module.
Reference: GreenGate – A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis.
Verification: None
Depositor(s): Jan Lohmann
Catalog ID: 48866
Product details
Bacteria Resistance: Chloramphenicol and Gentamycin
Vector backbone: pGreenII
Vector type: Golden Gate compatible cloning vector
Backbone size without insert: 2492 bp
Copy number: High
Growth strain: DB3.1
Growth temperature: 37°C
Antibiotics Resistance: Unknown
Depositor comment: This plasmid is designed as destination vector for Golden Gate cloning using the BsaI enzyme. The 5′ BsaI overhang can be exchanged using KpnI and EcoRI. The 3′ BsaI overhang can be exchanged using XhoI and XbaI. There is also a a NotI-PacI-SgsI-SfaAI-KpnI MCS in front of the A-overhang and an XbaI-PaeI MCS after the G-overhang.
Please note that a transposon insertion is present in the chloramphenicol resistance promoter. This insertion does not interfere with plasmid function. There is an additional BsaI site at bp# 1889-1894 which is irrelevant for GreenGate reactions with this plasmid.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 64-65bp
transposon integration = 747-277bp
BsaI site #2 = 3414-3419bp
Please note that a transposon insertion is present in the chloramphenicol resistance promoter. This insertion does not interfere with plasmid function. There is an additional BsaI site at bp# 1889-1894 which is irrelevant for GreenGate reactions with this plasmid.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 64-65bp
transposon integration = 747-277bp
BsaI site #2 = 3414-3419bp
Gene / Insert: None
