pGGF008
Properties and application
Provides a BASTA resistance cassette as GreenGate module.
MGLCC accession number
48848
RM350.00
Purified plasmid
Product Information
| Vector backbone | pUC19 |
| Vector type | Golden Gate compatible cloning vector |
| Bacterial resistance | Ampicillin |
| Growth temperature | 37°C |
| Host range | DH5alpha |
| Copy number | High Copy |
| Cloned gene | pNOS::BastaR::tNOS |
| Cloned gene (alt name) | Basta resistance cassette |
| Gene size, bp | 1083 |
| Mutation | chi sequence in BastaR removed by silent substitution |
| Species | Synthetic |
| Comment | This plasmid is designed for Golden Gate cloning using the BsaI enzyme. Plasmid Features (listed as bp in full plasmid sequence): BsaI site #1 = 2241-2251bp BASTA resistance cassette with NOS promoter and terminator = 2252-3334bp BsaI site #2 = 3335-3345bp |
