Address
Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia
Work Hours
Monday to Friday: 830AM - 530PM
pGGF004
RM150.00
Purpose / Description: Provides the AP3 promoter as GreenGate module.
Reference: GreenGate – A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis.
Verification: None
Depositor(s): Jan Lohmann
Catalog ID: 48845
Product details
Bacteria Resistance: Ampicillin
Vector backbone: pUC19
Vector type: Golden Gate compatible cloning vector
Backbone size without insert: 2686 bp
Copy number: High
Growth strain: Mach1
Growth temperature: 37°C
Antibiotics Resistance: Unknown
Depositor comment: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
D-alanine resistance cassette with 35S promoter and terminator = 2252-4434bp
BsaI site #2 = 4435-4445bp
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
D-alanine resistance cassette with 35S promoter and terminator = 2252-4434bp
BsaI site #2 = 4435-4445bp
Gene / Insert: p35S::D-AlanineR::t35S (a.k.a. D-alanine resistance cassette)
Insert size: 2183 bp
Species: Synthetic
Mutation: internal BsaI recognition site in 35S promoter removed by substitution
