pGGF004
Properties and application
Provides a D-alanine resistance cassette as GreenGate module.
MGLCC accession number
48845
RM350.00
Purified plasmid
Product Information
| Vector backbone | pUC19 |
| Vector type | Golden Gate compatible cloning vector |
| Bacterial resistance | Ampicillin |
| Growth temperature | 37°C |
| Host range | Mach1 |
| Copy number | High Copy |
| Cloned gene | p35S::D-AlanineR::t35S |
| Cloned gene (alt name) | D-alanine resistance cassette |
| Gene size, bp | 2183 |
| Mutation | internal BsaI recognition site in 35S promoter removed by substitution |
| Species | Synthetic |
| Comment | This plasmid is designed for Golden Gate cloning using the BsaI enzyme. Plasmid Features (listed as bp in full plasmid sequence): BsaI site #1 = 2241-2251bp D-alanine resistance cassette with 35S promoter and terminator = 2252-4434bp BsaI site #2 = 4435-4445bp |
