pDGE Dicot Genome Editing Kit

Introduction

Golden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants. Please Note: Plasmids pDGE144-169 are not functional for genome editing in Arabidopsis thaliana.

MGLCC accession number
Kit #1000000084

RM750.00

Description

This collection was designed for the simple Golden Gate cloning-based assembly of constructs for genome editing in dicotyledonous plants. The collection consists of “genome editing recipient” and “sgRNA shuttle” vectors, respectively. Recipient vectors are Agrobacterium-compatible, and contain a Cas9 expression cassette, a plant-selectable marker and a /ccdB/ cassette as a “dummy”. Shuttle vectors allow the generation of sgRNA transcriptional units from annealed oligonucleotides. sgRNA transcriptional units are then mobilized into recipient vectors, replacing the /ccdB/ dummy.

Accession NumberNameProperties And Applicationhf:categories
Kit #1000000084pDGE Dicot Genome Editing Kitkit
79440pDGE1recipient plasmid [multiplexing]; p35S:Cas9, nptIIplasmid
79466pDGE10sgRNA shuttle vector; module 4plasmid
79467pDGE11sgRNA shuttle vector; module 4Eplasmid
79469pDGE12sgRNA shuttle vector; module 5plasmid
79471pDGE13sgRNA shuttle vector; module 6plasmid
79473pDGE14sgRNA shuttle vector; module 7plasmid
79448pDGE144recipient plasmid [multiplexing]; p35S:Cas9, FAST, BASTAplasmid
79449pDGE146recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, BASTAplasmid
79450pDGE148recipient plasmid [multiplexing]; pDD45:Cas9, FAST, BASTAplasmid
79474pDGE15sgRNA shuttle vector; module 8Eplasmid
79451pDGE151recipient plasmid [multiplexing]; pICU2:Cas9, FAST, BASTAplasmid
79452pDGE160recipient plasmid [multiplexing]; p35S:Cas9, FAST, nptIIplasmid
79453pDGE161recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, nptIIplasmid
79454pDGE164recipient plasmid [multiplexing]; pDD45:Cas9, FAST, nptIIplasmid
79455pDGE165recipient plasmid [multiplexing]; pICU2:Cas9, FAST, nptIIplasmid
79456pDGE166recipient plasmid [multiplexing]; p35S:Cas9, FAST, Hygroplasmid
79457pDGE167recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, Hygroplasmid
79458pDGE168recipient plasmid [multiplexing]; pDD45:Cas9, FAST, Hygroplasmid
79459pDGE169recipient plasmid [multiplexing]; pICU2:Cas9, FAST, Hygroplasmid
79441pDGE2recipient plasmid [multiplexing]; p35S:Cas9, BASTAplasmid
79442pDGE3recipient plasmid [multiplexing]; pPcUbi:Cas9, nptIIplasmid
79443pDGE4recipient plasmid [multiplexing]; pPcUbi:Cas9, BASTAplasmid
79461pDGE5sgRNA shuttle vector; module 1plasmid
79476pDGE6sgRNA shuttle vector; module 1Eplasmid
79444pDGE62recipient plasmid [single nuclease]; p35S:Cas9, nptIIplasmid
79445pDGE63recipient plasmid [single nuclease]; p35S:Cas9, BASTAplasmid
79446pDGE64recipient plasmid [single nuclease]; pPcUbi:Cas9, nptIIplasmid
79447pDGE65recipient plasmid [single nuclease]; pPcUbi:Cas9, BASTAplasmid
79462pDGE7sgRNA shuttle vector; module 2plasmid
80578pDGE76recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptIIplasmid
80579pDGE77recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTAplasmid
80580pDGE78recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptIIplasmid
80581pDGE79recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTAplasmid
79463pDGE8sgRNA shuttle vector; module 2Eplasmid
79465pDGE9sgRNA shuttle vector; module 3plasmid
80582pJOG250recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptIIplasmid
80583pJOG251recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTAplasmid
80584pJOG252recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptIIplasmid
80585pJOG253recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTAplasmid
80586pJOG292(Empty Backbone) empty vector (lacZ) for 2nd generation recipient assemblyplasmid
80587pJOG294dummy module (ccdB) for 2nd generation recipient assemblyplasmid