pJOG251
Properties and application
recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA
MGLCC accession number
80583
RM350.00
Purified plasmid
Product Information
| Vector backbone | pVM_BGW-BpiI |
| Vector type | Plant Expression, CRISPR |
| Bacterial resistance | Chloramphenicol and Spectinomycin |
| Growth temperature | 37°C |
| Host range | ccdB Survival |
| Copy number | High Copy |
| Cloned gene | GENE/INSERT 1: pnos:PAT-tnos GENE/INSERT 2: p35S:Cas9 D10A/N863A-Hax3(CT)-t35S GENE/INSERT 3: BsaI-ccdB_CmR-BsaI |
| Gene size, bp | GENE/INSERT 1: 1800 GENE/INSERT 2: 5800 GENE/INSERT 3: 1500 |
| Species | GENE/INSERT 1: E. coli GENE/INSERT 2: Streptococcus pyogenes |
| Mutation | GENE/INSERT 2: D10A |
| Entrez gene | - |
| Comment | Please note- Functional data on this plasmid can be found in the following publication: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations. Streubel et al PLoS One. 2017 Mar 16;12(3):e0173580. PMID: 28301511 |
