Address
Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia
Work Hours
Monday to Friday: 830AM - 530PM
pJOG250
RM150.00
Purpose / Description: recipient plasmid [multiplexing, activator]; p35S:Cas9 D1A/N863A-Hax3CT, nptII
Reference: Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit.
Verification: None
Depositor(s): Johannes Stuttmann
Catalog ID: 80582
Product details
Bacteria Resistance: Chloramphenicol and Spectinomycin
Vector backbone: pVM_BGW
Vector type: Plant Expression, CRISPR
Total vector size: 15900 bp
Copy number: High
Growth strain: ccdB Survival
Growth temperature: 37°C
Antibiotics Resistance: Unknown
Depositor comment: Please note- Functional data on this plasmid can be found in the following publication: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations. Streubel et al PLoS One. 217 Mar 16;12(3):e17358. PMID: 2831511
Gene / Insert: Gene 1: pnos:nptII-tnos; 18bp; E. coli || Gene 2: p35S:Cas9 D1A/N863A-Hax3(CT)-t35S; 58bp; Streptococcus pyogenes || Gene 3: BsaI-ccdB_CmR-BsaI; 15bp
Mutation: D1A
