FX Cloning System
The 35 plasmid FX cloning kit can be used to insert a sequence of interest into a cloning vector and easily swap into expression vectors for several organisms with a variety of N- and C-terminal tags.
RM330.00
The FX cloning system (fragment exchange cloning) is an efficient, inexpensive cloning method that utilizes a Type IIS restriction enzyme and negative selection markers. This system is suitable for both prokaryotic and eukaryotic expression systems and combines the advantages of established recombination- and ligation-independent cloning methods, while reducing undesired cloning-related sequences added in the process.
The FX cloning system uses a set of three initial cloning vectors (pINIT_cat, pINIT_kan and pINIT_tet) for the initial cloning of a PCR product into one of the vectors, which differ by bacterial resistances. The subcloning of the ORF from the pINITIAL vector into an expression vector utilizes the same cloning procedure. PCR products can also be directly cloned into expression vectors.
The FX cloning Kit contains 3 initial cloning vectors, 12 E. coli expression vectors, 3 L. lactis expression vectors, 4 P. pastoris expression vectors, 4 S. cerevisiae expression vectors, 4 mammalian expression vectors, 2 Xenopus oocyte expression vectors and 3 insect expression vectors. A variety of expression vectors are available containing several combinations of 3C protease-cleavable N-terminal and C-terminal tags , including decaHis, Myc, Streptavidin Binding Peptide and Avi tags plus GFP.
| Accession Number | Name | Properties And Application | hf:categories |
|---|---|---|---|
| Kit #1000000039 | FX Cloning System | kit | |
| 47066 | p7XC3GH | (Empty Backbone)?FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags | plasmid |
| 47065 | p7XC3H | (Empty Backbone) FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tag | plasmid |
| 47064 | p7XNH3 | (Empty Backbone)?FX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage site | plasmid |
| 47070 | pBXC3GH | (Empty Backbone)?FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags | plasmid |
| 47068 | pBXC3H | (Empty Backbone) FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag | plasmid |
| 47071 | pBXCA3GH | (Empty Backbone) FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease cleavage site, GFP and 10x His tags | plasmid |
| 47072 | pBXCA3H | (Empty Backbone)?FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag | plasmid |
| 47073 | pBXNAC3GH | (Empty Backbone)?FX cloning E.coli expression vector with araBAD promoter, N-terminal Avi-tag, C-terminal 3C protease cleavage site, GFP and 10x His tags | plasmid |
| 47074 | pBXNAC3H | (Empty Backbone)?FX cloning E.coli expression vector with araBAD promoter N-terminal Avi-tag, C-terminal 3C protease cleavage site and 10x His tag | plasmid |
| 47067 | pBXNH3 | (Empty Backbone) FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His tag and 3C protease cleavage site | plasmid |
| 47075 | pBXNH3A | (Empty Backbone)?FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His, 3C protease cleavage site and Avi-tag | plasmid |
| 47069 | pBXNH3CA | (Empty Backbone)?FX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tag | plasmid |
| 49031 | pcDXC3GMS | (Empty Backbone)?FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide | plasmid |
| 49030 | pcDXC3MS | (Empty Backbone)?FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, Myc tag and streptavidin binding peptide | plasmid |
| 49028 | pcDXNSM3 | (Empty Backbone) FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag and 3C protease cleavage site | plasmid |
| 49029 | pcDXNSMG3 | (Empty Backbone)?FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag, EGFP and 3C protease cleavage site | plasmid |
| 49051 | pFBXC3GH | (Empty Backbone)?FX cloning insect cell expression vector | plasmid |
| 49050 | pFBXC3H | (Empty Backbone)?FX cloning insect cell expression vector | plasmid |
| 49049 | pFBXNH3 | (Empty Backbone)?FX cloning insect cell expression vector | plasmid |
| 49023 | pICXC3GH | (Empty Backbone)?FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag | plasmid |
| 49022 | pICXC3H | (Empty Backbone)?FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag | plasmid |
| 49020 | pICXNH3 | (Empty Backbone)?FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site | plasmid |
| 49021 | pICXNHG3 | (Empty Backbone) FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site | plasmid |
| 46858 | pINIT_cat | (Empty Backbone)?FX sequencing vector for subcloning, chloramphenicol marker | plasmid |
| 46973 | pINIT_kan | (Empty Backbone)?FX sequencing vector for subcloning, kanamycin marker | plasmid |
| 46974 | pINIT_tet | (Empty Backbone)?FX sequencing vector for subcloning, tetracycline marker | plasmid |
| 47077 | pREXC3GH | (Empty Backbone)?FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags | plasmid |
| 47078 | pREXC3H | (Empty Backbone)?FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site and 10x His tags | plasmid |
| 47079 | pREXNH3 | (Empty Backbone)?FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and N-terminal 10x His tag and 3C protease cleavage site | plasmid |
| 49032 | pTLNX | (Empty Backbone)?FX cloning Xenopus oocyte expression vector with SP6 promoter | plasmid |
| 49033 | pTLNXA7 | (Empty Backbone)?FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence | plasmid |
| 49027 | pYEXC3GH | (Empty Backbone)?FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag | plasmid |
| 49026 | pYEXC3H | (Empty Backbone)?FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tag | plasmid |
| 49024 | pYEXNH3 | (Empty Backbone)?FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site | plasmid |
| 49025 | pYEXNHG3 | (Empty Backbone)?FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site | plasmid |