Address
Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia
Work Hours
Monday to Friday: 830AM - 530PM
pYEXC3GH
RM150.00
Purpose / Description: (Empty Backbone) FX cloning E.coli expression vector with araBAD promoter and N-terminal 1x His tag and 3C protease cleavage site
Reference: A versatile and efficient high-throughput cloning tool for structural biology.
Verification: None
Depositor(s): Raimund Dutzler
Catalog ID: 49027
Product details
Bacteria Resistance: Chloramphenicol and Ampicillin
Vector backbone: pYES2/CT
Vector type: Yeast Expression
Backbone size without insert: 8362 bp
Total vector size: 8362 bp
Backbone Manufacturer: Invitrogen
Modification to backbone: modified for compatibility with FX cloning and added tags/fusion proteins
Copy number: Low
Growth strain: DB3.1
Growth temperature: 37°C
Antibiotics Resistance: Unknown
Depositor comment: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland. The ampicillin resistance cassette is located at bp# 4361-5221.
Gene / Insert: None
Cloning method: Unknown
Selectable marker: URA3
Promoter: GAL1
Tag / fusion protein: 3C protease site (PreScission site) (C terminal on backbone); GFP (C terminal on backbone); decaHIS (1x His) (C terminal on backbone)
5′ sequencing primer: GAL1
3′ sequencing primer: CYC1
