Address
Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia
Work Hours
Monday to Friday: 830AM - 530PM
pcDXC3GMS
RM150.00
Purpose / Description: (Empty Backbone) FX cloning E.coli expression vector with araBAD promoter and N-terminal 1x His tag and 3C protease cleavage site
Reference: A versatile and efficient high-throughput cloning tool for structural biology.
Verification: None
Depositor(s): Raimund Dutzler
Catalog ID: 49031
Product details
Bacteria Resistance: Chloramphenicol and Ampicillin
Vector backbone: pcDNA3.1
Vector type: Mammalian Expression
Total vector size: 6578 bp
Backbone Manufacturer: Invitrogen
Modification to backbone: modified for compatibility with FX cloning and added tags/fusion proteins
Copy number: High
Growth strain: DB3.1
Growth temperature: 37°C
Antibiotics Resistance: Unknown
Depositor comment: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Gene / Insert: None
Cloning method: Unknown
Promoter: CMV
Tag / fusion protein: 3C protease site (PreScission site) (C terminal on backbone); EGFP (C terminal on backbone); MYC (C terminal on backbone); streptavidin binding peptide (SBP) (C terminal on backbone)
5′ sequencing primer: CMV-F
3′ sequencing primer: BGH-rev
