Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia

Work Hours
Monday to Friday: 830AM - 530PM



Purpose / Description: (Empty Backbone) FX cloning E.coli expression vector with araBAD promoter and N-terminal 1x His tag and 3C protease cleavage site
Reference: A versatile and efficient high-throughput cloning tool for structural biology.
Verification: None
Depositor(s): Raimund Dutzler
Catalog ID: 49022

Product details

Bacteria Resistance: Chloramphenicol and Bleocin (Zeocin)
Vector backbone: pPICZ
Vector type: Yeast Expression
Total vector size: 5050 bp
Backbone Manufacturer: Invitrogen
Modification to backbone: modified for compatibility with FX cloning and added tags/fusion proteins
Copy number: Low
Growth strain: DB3.1
Growth temperature: 37°C
Antibiotics Resistance: Unknown
Depositor comment: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Gene / Insert: None
Cloning method: Unknown
Selectable marker: Zeocin
Promoter: AOX1
Tag / fusion protein: 3C protease site (PreScission site) (C terminal on backbone); decaHis (1x His) (C terminal on backbone)
5′ sequencing primer: AOX1-fwd (‘5-GACTGGTTCCAATTGACAAGC-3’)
3′ sequencing primer: AOX1-rev (5′-GCAAATGGCATTCTGACATCC-3′)