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Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia

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pGDP3 arr

RM150.00

Purpose / Description: Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
Reference: A Common Platform for Antibiotic Dereplication and Adjuvant Discovery.
Verification: None
Depositor(s): Gerard Wright
Catalog ID: 112890

Product details

Bacteria Resistance: Ampicillin
Vector backbone: pBR322
Vector type: Bacterial Expression
Copy number: Low
Growth strain: DH5alpha
Growth temperature: 37°C
Antibiotics Resistance: Unknown
Depositor comment: JoVE antibiotic dereplication paper: Zubyk, H.L., Cox, G., Wright, G.D. Antibiotic Dereplication Using the Antibiotic Resistance Platform. J. Vis. Exp. (152), e6536, doi:1.3791/6536 (219). (https://www.jove.com/video/6536/antibiotic-dereplication-using-the-antibiotic-resistance-platform)
Gene / Insert: arr
Cloning method: Restriction Enzyme
5′ cloning site: unknown (unknown if destroyed)
Promoter: Pbla
Tag / fusion protein: 6xHis (N terminal on backbone)
5′ sequencing primer: CATGAGACAATAACCCTG
3′ sequencing primer: CCAACTCAGCTTCCTTTC