Address
Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia
Work Hours
Monday to Friday: 830AM - 530PM
pGDP1 uvrA
RM150.00
Purpose / Description: Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
Reference: A Common Platform for Antibiotic Dereplication and Adjuvant Discovery.
Verification: None
Depositor(s): Gerard Wright
Catalog ID: 112892
Product details
Bacteria Resistance: Kanamycin
Vector backbone: pBR322
Vector type: Bacterial Expression
Copy number: Low
Growth strain: DH5alpha
Growth temperature: 37°C
Antibiotics Resistance: Unknown
Depositor comment: uvrA gene was taken from the Echinomycin biosynthetic gene cluster. JoVE antibiotic dereplication paper: Zubyk, H.L., Cox, G., Wright, G.D. Antibiotic Dereplication Using the Antibiotic Resistance Platform. J. Vis. Exp. (152), e6536, doi:1.3791/6536 (219). (https://www.jove.com/video/6536/antibiotic-dereplication-using-the-antibiotic-resistance-platform)
Gene / Insert: uvrA-like protein
Cloning method: Restriction Enzyme
5′ cloning site: NdeI (not destroyed)
3′ cloning site: HindIII (not destroyed)
Promoter: Pbla
Tag / fusion protein: 6xHis (N terminal on backbone)
5′ sequencing primer: CATGAGACAATAACCCTG
3′ sequencing primer: CCAACTCAGCTTCCTTTC
