pGDP1 vph

Properties and application
This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
Depositor
Gerard Wright
MGLCC accession number
112893

RM350.00

Purified plasmid

Product Information
Bacterial resistanceKanamycin
Host rangeDH5alpha
Growth temperature37°C
Vector backbonepBR322
Vector typeBacterial Expression
Copy numberLow copy
Cloning methodRestriction Enzyme
5' cloning siteNdeI (not destroyed)
3' cloning siteXhoI (not destroyed)
5' sequencing primerCATGAGACAATAACCCTG
3' sequencing primerCCAACTCAGCTTCCTTTC
PromoterPbla
Tag / fusion protein6xHis (N terminal on backbone)
Cloned genevph
Cloned gene (alt name)-
SpeciesStreptomyces vinaceus
Entrez gene-
Sequence detail> vph sequence ATGAGAATCATTGAGACGCACCGCGATCTGCTGAGCCGGCTCCTGCCCGGGGACACCGTGGGCGGACTCGCCGTCCACGAGGGGCAGTTCCACCATGTGGTGATCGGATCGCACCGGGTGGTCTGCTTCGCCCGCACCCGGGCGGCCGCCGACCGTCTGCCCGGCAGGGCGGACGTCCTGCGCGCTCTTGCCGGGATCGACCTCGGGTTTCGCACGCCGCAGCCGCTGTCCGAGGGCGGCGCCCAGGGCACGGACGAGCCGCCGTACCTGGTGCTGAGCCGCATTCCCGGAGCACCGTTGGAGGACGATGTGCTCACCAGCCCGGAGGTGGCGGAGGCCGTCGCCCGACAGTACGCGACCCTGCTGTCCGGGCTCGCGGCGGCGGGCGACGAGGAGAAGGTGCGCGCCGCGCTGCCGGAGGCTCCCGCGAACGAGTGGCAGGAGTTCGCCACGGGGGTGCGTACCGAACTGTTCCCGCTGATGTCCGACGGCGGCCGGGAGCGTGCCGAGCGCGAGCTCGCCGCGCTCGACGCCCTGCCCCATCTCACCTCCGCGGTGGTCCACGGTGACCTCGGCGGCGAGAACGTCCTGTGGGAGACGGTGGACGGAGTGCCGCGCATGAGCGGCGTCGTCGACTGGGACGAGGTCGGCATCGGCGACCCGGCTGAGGACCTGGCCGCCATCGGGGCGAGCTACGGCGAGGAACTGCTGGGCCGAGTGCTCGCGCTCGGCGGCTGGGCCGACAACGGAACGGCCGAGCGGATCTCGGCGATCCGAGGCACCTTCGCACTCCAGCAGGCCCTCTACGCGCAGCGCGACGGCGACGAGGAGGAACTCGCCGACGGCCTCAGCGGCTACCGGTAGG