pWZL Neo Myr Flag PRKAA1
Properties and application
This plasmid is part of the Myristoylated Kinase Library Kit.
MGLCC accession number
20595
RM350.00
Purified plasmid
Product Information
| Vector backbone | pWZL-Neo-Myr-Flag-DEST |
| Vector type | Mammalian Expression, Retroviral |
| Bacterial resistance | Ampicillin |
| Growth temperature | 37°C |
| Host range | Stbl3 |
| Cloning method | Gateway Cloning |
| 5' sequencing primer | pWZL-Fwd (GAA CCT CCT CGT TCG ACC) |
| 3' sequencing primer | pWZL-Rev (TTC CGG GCC CTC ACA TTG) |
| Cloned gene | PRKAA1 |
| Cloned gene (alt name) | protein kinase, AMP-activated, alpha 1 catalytic subunit |
| GenBank ID | - |
| Species | H. sapiens (human) |
| Mutation | - |
| Entrez gene | PRKAA1 (a.k.a. AMPK, AMPKa1) |
| Tag / fusion protein | Myr (N terminal on backbone) Flag (N terminal on backbone) |
| Comment | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 Addgene's sequencing results suggest that the best reference sequence for the PRKAA1 insert in this plasmid is AB022017.1. |
