pGDP3 aph(3”)-Ia

Properties and application
This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
Depositor
Gerard Wright
MGLCC accession number
112879

RM350.00

Purified plasmid

Product Information
Bacterial resistanceAmpicillin
Host rangeDH5alpha
Growth temperature37°C
Vector backbonepBR322
Vector typeBacterial Expression
Copy numberLow copy
Cloning methodRestriction Enzyme
5' cloning siteNdeI (not destroyed)
3' cloning siteHindIII (not destroyed)
5' sequencing primerCATGAGACAATAACCCTG
3' sequencing primerCCAACTCAGCTTCCTTTC
PromoterPbla
Tag / fusion protein6xHis (N terminal on backbone)
Cloned geneaph(3'')-Ia
Cloned gene (alt name)aphD2, aphE
SpeciesStreptomyces griseus
Entrez geneSGR_RS01140 (a.k.a. SGR_RS01140, SGR_249)
Sequence detail> aph(3'')-Ia sequence ATGAGTGATCACCCCGGGCCGGGGGCCGTCACGCCGGAGCTGTTCGGCGTGGGCGGCGACTGGCTGGCCGTCACCGCGGGCGAATCGGGCGCCTCCGTCTTTCGCGCCGCGGACGCCACCCGGTACGCCAAGTGCGTGCCCGCCGCGGACGCGGCCGGTCTTGAGGCGGAACGCGACCGGATCGCCTGGCTGAGCGGGCAGGGCGTACCGGGCCCCCGCGTCCTCGACTGGTACGCCGGTGACGCGGGCGCCTGCCTGGTCACCCGTGCCGTCCCCGGCGTACCCGCTGATCGGGTGGGCGCCGATGACCTTCGCACTGCCTGGGGGGCCGTCGCGGACGCGGTCCGTCGGCTGCACGAGGTGCCCGTGGCCTCGTGTCCGTTCCGCCGGGGGCTGGACTCCGTGGTCGACGCCGCCCGTGACGTGGTGGCCCGTGGCGCGGTGCATCCGGAGTTCCTGCCGGTGGAGCAGCGGCTCGTTCCCCCGGCGGAGCTGCTGGCCCGGCTCACCGGGGAGCTCGCCCGTCGGCGCGATCAGGAGGCCGCCGACACGGTCGTCTGCCACGGTGATCTCTGCCTGCCCAACATCGTCCTCCATCCGGAGACCCTGGAGGTGTCGGGCTTCATCGACCTGGGACGGCTCGGGGCGGCCGACCGCCACGCCGACCTGGCGCTGCTGCTGGCCAACGCGCGCGAGACCTGGGTGGACGAGGAGCGGGCGCGGTTCGCCGACGCGGCGTTCGCCGAGCGTTACGGCATCGCCCCGGACCCGGAACGGCTGCGCTTCTACCTCCATCTCGATCCGCTCACCTGGGGCTAG