pWZL Neo Myr Flag RET
Properties and application
This plasmid is part of the Myristoylated Kinase Library Kit.
MGLCC accession number
20614
RM350.00
Purified plasmid
Product Information
| Vector backbone | pWZL-Neo-Myr-Flag-DEST |
| Vector type | Mammalian Expression, Retroviral |
| Bacterial resistance | Ampicillin |
| Growth temperature | 37°C |
| Host range | Stbl3 |
| Cloning method | Gateway Cloning |
| 5' sequencing primer | pWZL-Fwd (GAA CCT CCT CGT TCG ACC) |
| 3' sequencing primer | pWZL-Rev (TTC CGG GCC CTC ACA TTG) |
| Cloned gene | RET |
| Cloned gene (alt name) | Alt name: ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease) Alt name: RET9 |
| GenBank ID | NM_020630 |
| Species | H. sapiens (human) |
| Mutation | R982C |
| Entrez gene | RET (a.k.a. CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET-ELE1) |
| Tag / fusion protein | Myr (N terminal on backbone) Flag (N terminal on backbone) |
| Comment | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Addgene's sequencing results identified a R982C mutation when compared to GenBank ID NP_065681.1. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 |
