pWZL Neo Myr Flag NEK6

Properties and application
This plasmid is part of the Myristoylated Kinase Library Kit.
Depositor
William Hahn
MGLCC accession number
20548

RM350.00

Purified plasmid

Product Information
Vector backbonepWZL-Neo-Myr-Flag-DEST
Vector typeMammalian Expression, Retroviral
Bacterial resistanceAmpicillin
Growth temperature37°C
Host rangeStbl3
Cloning methodGateway Cloning
5' sequencing primerpWZL-Fwd (GAA CCT CCT CGT TCG ACC)
3' sequencing primerpWZL-Rev (TTC CGG GCC CTC ACA TTG)
Cloned geneNEK6
Cloned gene (alt name)NIMA (never in mitosis gene a)-related kinase 6
GenBank IDNM_014397
SpeciesH. sapiens (human)
Mutation-
Entrez geneNEK6 (a.k.a. SID6-1512)
Tag / fusion proteinMyr (N terminal on backbone) Flag (N terminal on backbone)
CommentORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021