pGGF012
Properties and application
Provides a sulfadiazine resistance cassette as GreenGate module.
MGLCC accession number
48849
RM350.00
Purified plasmid
Product Information
| Vector backbone | pUC19 |
| Vector type | Golden Gate compatible cloning vector |
| Bacterial resistance | Ampicillin |
| Growth temperature | 37°C |
| Host range | XL1 Blue |
| Copy number | High Copy |
| Cloned gene | pMAS::SulfadiazineR::t35S |
| Cloned gene (alt name) | sulfadiazine resistance cassett |
| Gene size, bp | 1631 |
| Mutation | internal BsaI recognition sites in SulfR removed by silent substitutions |
| Species | Synthetic |
| Comment | This plasmid is designed for Golden Gate cloning using the BsaI enzyme. Plasmid Features (listed as bp in full plasmid sequence): BsaI site #1 = 2241-2251bp Sulfadiazine resistance cassette with MAS promoter and 35S terminator = 2252-3882bp BsaI site #2 = 3883-3893bp |
