EKAR2G_design1_mTFP_227_Venus_173
Properties and application
This plasmid is part of the cpFRET - FRET based biosensors kit.
MGLCC accession number
39835
RM350.00
Purified plasmid
Product Information
| Bacterial resistance | Ampicillin |
| Host range | Top10 |
| Growth temperature | 37°C |
| Vector backbone | pTriEx4 |
| Vector type | Mammalian Expression, Bacterial Expression, Insect Expression |
| Vector size, bp | 7050 |
| Copy number | High copy |
| Cloning method | Restriction Enzyme |
| 5' cloning site | PacI (not destroyed) |
| 3' cloning site | Xhol (not destroyed) |
| 5' sequencing primer | ctggttattgtgctgtctc |
| 3' sequencing primer | tcagtggtatttgtga |
| Promoter | CMV |
| Tag / fusion protein | His-Myc (N terminal on insert) |
| Cloned gene | Erk activity reporter |
| Gene size, bp | 2189 |
| Species | Synthetic |
| Comment | A genetically encoded fluorescent sensor of ERK activity. 2008. PNAS 205(49) |
