Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia

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pWZL Neo Myr Flag PDK1


Purpose / Description: CKS1B (GenBank ID: NM_1826)
Reference: Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene.
Verification: None
Depositor(s): William Hahn
Catalog ID: 20564

Product details

Bacteria Resistance: Ampicillin
Vector backbone: pWZL-Neo-Myr-Flag-DEST
Vector type: Mammalian Expression, Retroviral
Copy number: Unknown
Growth strain: Stbl3
Growth temperature: 37°C
Antibiotics Resistance: Unknown
Depositor comment: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5′ myristoylation and flag tags.

This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library
Gene / Insert: PDK1 (GenBank ID: NM_261)
Entrez gene: PDK1
Species: H. sapiens (human)
Cloning method: Gateway Cloning
Selectable marker: Neomycin (select with G418)
Tag / fusion protein: Myr (N terminal on backbone); Flag (N terminal on backbone)
5′ sequencing primer: pWZL-Fwd (GAA CCT CCT CGT TCG ACC)
3′ sequencing primer: pWZL-Rev (TTC CGG GCC CTC ACA TTG)