pJOG587

MGLCC accession number
105431

RM350.00

Purified plasmid

Product Information

Vector backbonepICH41264 (Engler et al., 2014)
Vector typeSynthetic Biology
Bacterial resistanceSpectinomycin
Growth temperature37°C
Host rangeDH5alpha
Copy numberHigh Copy
Cloning methodRestriction Enzyme
5' cloning siteBpiI (destroyed during cloning)
3' cloning siteBpiI (destroyed during cloning)
5' sequencing primerCTTTGAGTGAGCTGATACCG
3' sequencing primerGGGTTCCGCGCACATTTC
Cloned genebeta-glucuronidase (2 introns; no ATG-Start codon)
Species-
MutationBsaI/ BpiI restriction sites removed