pGDP3 vatD

Properties and application
This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
Depositor
Gerard Wright
MGLCC accession number
112885

RM350.00

Purified plasmid

Product Information
Bacterial resistanceAmpicillin
Host rangeDH5alpha
Growth temperature37°C
Vector backbonepBR322
Vector typeBacterial Expression
Copy numberLow copy
Cloning methodRestriction Enzyme
5' cloning siteunknown (unknown if destroyed)
3' cloning site-
5' sequencing primerCATGAGACAATAACCCTG
3' sequencing primerCCAACTCAGCTTCCTTTC
PromoterPbla
Tag / fusion protein-
Cloned genevatD
Cloned gene (alt name)vat(D), satA
SpeciesEnterococcus faecium
Entrez gene-
Sequence detail> vatD sequence ATGGGTCCGAATCCTATGAAAATGTATCCTATAGAAGGAAACAAATCAGTACAATTTATCAAACCTATTT TAGAAAAATTAGAAAATGTTGAGGTTGGAGAATACTCATATTATGATTCTAAGAATGGAGAAACTTTTGA TAAGCAAATTTTATATCATTATCCAATCTTAAACGATAAGTTAAAAATAGGTAAATTTTGCTCAATAGGA CCAGGTGTAACTATTATTATGAATGGAGCAAATCATAGAATGGATGGCTCAACATATCCATTTAATTTAT TTGGTAATGGATGGGAGAAACATATGCCAAAATTAGATCAACTACCTATTAAGGGGGATACAATAATAGG TAATGATGTATGGATAGGAAAAGATGTTGTAATTATGCCAGGAGTAAAAATCGGGGATGGTGCAATAGTA GCTGCTAATTCTGTTGTTGTAAAAGATATAGCGCCATACATGTTAGCTGGAGGAAATCCTGCTAACGAAA TAAAACAAAGATTTGATCAAGATACAATAAATCAGCTGCTTGATATAAAATGGTGGAATTGGCCAATAGA CATTATTAATGAGAATATAGATAAAATTCTTGATAATAGCATCATTAGAGAAGTCATATGGAAAAAATGA