pGDP1 NDM-1
Properties and application
This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
MGLCC accession number
112883
RM350.00
Purified plasmid
Product Information
| Bacterial resistance | Kanamycin |
| Host range | DH5alpha |
| Growth temperature | 37°C |
| Vector backbone | pBR322 |
| Vector type | Bacterial Expression |
| Copy number | Low copy |
| Cloning method | Restriction Enzyme |
| 5' cloning site | unknown (unknown if destroyed) |
| 3' cloning site | - |
| 5' sequencing primer | CATGAGACAATAACCCTG |
| 3' sequencing primer | CCAACTCAGCTTCCTTTC |
| Promoter | Pbla |
| Tag / fusion protein | 6xHis (N terminal on backbone) |
| Cloned gene | NDM-1 |
| Cloned gene (alt name) | - |
| Species | Klebsiella pneumoniae |
| Entrez gene | blaNDM-1 (a.k.a. D647_p47098) |
| Sequence detail | > NDM-1 sequence GCATTGATGCTGAGCGGGTGCATGCCCGGTGAAATCCGCCCGACGATTGGCCAGCAAATGGAAACTGGCGACCAACGGTTTGGCGATCTGGTTTTCCGCCAGCTCGCACCGAATGTCTGGCAGCACACTTCCTATCTCGA CATGCCGGGTTTCGGGGCAGTCGCTTCCAACGGTTTGATCGTCAGGGATGGCGGCCGCGTGCTGGTGGTCGATACCGCCTGGACCGATGACCAGACCGCCCAGATCCTCAACTGGATCAAGCAGGAGATCAACCTGCCGGTCGCGCTGGCGGTGGTGACTCACGCGCATCAGGACAAGATGGGCGGTATGGACGCGCTGCATGCGGCGGGGATTGCGACTTATGCCAATGCGTTGTCGAACCAGCTTGCCCCGCAAGAGGGGATGGTTGCGGCGCAACACAGCCTGACTTTCGCCGCCAATGGCTGGGTCGAACCAGCAACCGCGCCCAACTTTGGCCCGCTCAAGGTATTTTACCCCGGCCCCGGCCACACCAGTGACAATATCACCGTTGGGATCGACGGCACCGACATCGCTTTTGGTGGCTGCCTGATCAAGGACAGCAAGGCCAAGTCGCTCGGCAATCTCGGTGATGCCGACACTGAGCACTACGCCGCGTCAGCGCGCGCGTTTGGTGCGGCGTTCCCCAAGGCCAGCATGATCGTGATGAGCCATTCCGCCCCCGATAGCCGCGCCGCAATCACTC |
