MoClo Chlamydomonas Toolkit

Name: MoClo Chlamydomonas Toolkit
SKU: 4454
Availability: InStock

Depositor

Publication

Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii

MGLCC accession number

Kit #1000000013

RM800.00

Description

From Crozet et al. (2018): “… we have developed a Modular Cloning toolkit for the green microalga Chlamydomonas reinhardtii. It is based on Golden Gate cloning with standard syntax, and comprises 119 openly distributed genetic parts, most of which have been functionally validated in several strains. It contains promoters, UTRs, terminators, tags, reporters, antibiotic resistance genes, and introns cloned in various positions to allow maximum modularity. The toolkit enables rapid building of engineered cells for both fundamental research and algal biotechnology. This work will make Chlamydomonas the next chassis for sustainable synthetic biology.”

Accession Number	Name	Properties And Application	hf:categories
Kit #1000000013	MoClo Chlamydomonas Toolkit		kit microalgae
A20000	pCM0-001	Add PSAD promoter only (without 5'UTR)	plasmid
A20001	pCM0-002	Add HSP70A-RBSC2 tandem promoter	plasmid
A20002	pCM0-003	Add NIT1 promoter	plasmid
A20003	pCM0-004	METE promoter for inducible regulation of transgene expression with B12	plasmid
A20004	pCM0-006	strong constitutive promoter, no 5?UTR	plasmid
A20005	pCM0-007	constitutive under low light, induced by high light, no 5?UTR	plasmid
A20006	pCM0-008	Add HSP70A-RBSC2 tandem promoter, plus TSS and 22nt of RBCS2 5'UTR	plasmid
A20007	pCM0-009	Add NIT1 promoter with 5'UTR	plasmid
A20008	pCM0-010	Promoter Driving strong expression in Chlamy	plasmid
A20009	pCM0-011	Promoter Driving strong expression in Chlamy	plasmid
A20010	pCM0-012	Add NIT1 promoter plus 5'UTR	plasmid
A20011	pCM0-013	strong constitutive promoter, with 5?UTR	plasmid
A20012	pCM0-014	constitutive under low light, induced by high light, with 5?UTR	plasmid
A20013	pCM0-016	Promoter Driving strong expression in Chlamy	plasmid
A20014	pCM0-017	Promoter Driving strong expression in Chlamy	plasmid
A20015	pCM0-018	Add NIT1 promoter plus 5'UTR	plasmid
A20016	pCM0-019	METE promoter and 5?UTR for inducible regulation of transgene expression with B12	plasmid
A20017	pCM0-021	strong constitutive promoter, with 5?UTR	plasmid
A20018	pCM0-022	constitutive under low light, induced by high light, with 5?UTR	plasmid
A20019	pCM0-023	Add PSAD 5'UTR	plasmid
A20020	pCM0-024	Add RBCS2 5'UTR (strictly, no first intron)	plasmid
A20021	pCM0-025	Add NIT1 5'UTR	plasmid
A20022	pCM0-026	CrTHI4 riboswitch for inducible regulation of transgene expression	plasmid
A20023	pCM0-027	Add ?TUB2 5?UTR	plasmid
A20024	pCM0-028	Add VIPP2 5?UTR	plasmid
A20025	pCM0-029	Add PSAD 5'UTR	plasmid
A20026	pCM0-030	Add RBCS2 5'UTR (strictly, no first intron)	plasmid
A20027	pCM0-031	THI4 riboswitch (RS) for inducible regulation of transgene expression	plasmid
A20028	pCM0-032	Add NIT1 5'UTR	plasmid
A20029	pCM0-033	5?UTR	plasmid
A20030	pCM0-034	5?UTR	plasmid
A20031	pCM0-035	Add an ATG + 6*His tag in N-Terminus + a Gly	plasmid
A20032	pCM0-036	Add a StrepII tag in N-terminal	plasmid
A20033	pCM0-037	3*Myc-tag in N-terminus	plasmid
A20034	pCM0-038	N-terminal triple HA tag with Chlamy's codon usage and an internal Lys (after first HA)	plasmid
A20035	pCM0-039	N-terminal triple Flag tag with Chlamy's codon usage	plasmid
A20036	pCM0-042	Add a NanoLuc tag in N-terminal	plasmid
A20037	pCM0-045	Add a Clover (with RBCS2 intron 2) + StrepII tag in N-terminal	plasmid
A20038	pCM0-046	Add an mCerulean3 (with RBCS2 intron 2) + StrepII tag in N-terminal	plasmid
A20039	pCM0-047	Add an mRuby2 (with RBCS2 intron 2) + StrepII tag in N-terminal	plasmid
A20040	pCM0-048	Add a gLuc (with RBCS2 intron 2) + StrepII tag in N-terminal	plasmid
A20041	pCM0-049	Add an mVenus (with RBCS2 intron 2) + StrepII tag in N-terminal	plasmid
A20042	pCM0-050	red FP (RFP) with the intron 1 of RBCS2	plasmid
A20043	pCM0-051	Allows secretion to the extracellular matrix	plasmid
A20044	pCM0-052	Allows the targetting to the Chloroplast	plasmid
A20045	pCM0-053	Allows the targetting to the Chloroplast (CTP with intron)	plasmid
A20046	pCM0-054	Allows the targetting to the nucleus	plasmid
A20047	pCM0-055	Allows secretion to the extracellular matrix	plasmid
A20048	pCM0-056	Allows the targetting to ER (Nter)	plasmid
A20049	pCM0-057	Allows the targetting to the mitochondria (MTP with intron)	plasmid
A20050	pCM0-059	Allows expression of 2 proteines with same transcription (clivage during traduction)	plasmid
A20051	pCM0-061	NanoLuc in CDS without STOP and that can be fused in C-Ter	plasmid
A20052	pCM0-063	NanoLuc in CDS without STOP and that can be fused in C-Ter	plasmid
A20053	pCM0-066	mVenus (RbS2intron2) as Nter tag (Met/no stop)	plasmid
A20054	pCM0-067	red FP (RFP) with the intron 1 of RBCS2	plasmid
A20055	pCM0-068	Add a miR1157 precursor domesticated sequence with BpiI sites for cloning amiRNAs	plasmid
A20056	pCM0-069	Add a miR1157 precursor domesticated sequence with SpeI sites for cloning amiRNAs as in pChlamiRNA3	plasmid
A20057	pCM0-072	Ble(i) GFP fusion protein provinding resitance to Bleomycin or Zeocin	plasmid
A20058	pCM0-073	Resistance to Hygromycine in Chlamy	plasmid
A20059	pCM0-074	Resistance to Paromomycine in Chlamy	plasmid
A20060	pCM0-075	Gives Kanamycin resistance in Chlamydomonas	plasmid
A20061	pCM0-076	Confers resistance to Spectinomycine in Chlamy	plasmid
A20062	pCM0-077	provides resistance to Bleomycin or Zeocin (CDS with intron)	plasmid
A20063	pCM0-078	NanoLuc for testing promoters & terminators combinations	plasmid
A20064	pCM0-080	Add a Clover (with RBCS2 intron 2) + StrepII tag in CDS1	plasmid
A20065	pCM0-081	Add an mCerulean3 (with RBCS2 intron 2) + StrepII tag in CDS1	plasmid
A20066	pCM0-082	Add a gLuc (with RBCS2 intron 2) + StrepII tag in CDS1	plasmid
A20067	pCM0-084	mCherry2 (with RBCS2 intron1)	plasmid
A20068	pCM0-085	Add an mRuby2 (with RBCS2 intron 2) + StrepII tag in CDS1	plasmid
A20069	pCM0-086	Add an mVenus (with RBCS2 intron 2) + StrepII tag in CDS1	plasmid
A20070	pCM0-087	Allows expression of 2 proteines with same transcription (clivage during traduction)	plasmid
A20071	pCM0-089	Allows secretion to the extracellular matrix	plasmid
A20072	pCM0-090	Add a NanoLuc tag in c-terminal	plasmid
A20073	pCM0-093	C-terminal triple HA tag with Chlamydomonas codon usage and an internal Lys (after first HA)	plasmid
A20074	pCM0-094	C-terminal triple Flag tag with Chlamydomonas codon usage and a small sequence (AGAG) atthe beggining of the Flag tag, allowing a trypic finger printing of the protein (10 residues) even with a Lys at its end.	plasmid
A20075	pCM0-095	Add an 6*His tag in C-Terminus + a stop	plasmid
A20076	pCM0-096	Add a StrepII tag + STOP in C-terminal	plasmid
A20077	pCM0-097	3*Myc tags + STOP in C-terminal	plasmid
A20078	pCM0-099	allows affinity purification through Strep or by IMAC, tag + STOP in C-terminal	plasmid
A20079	pCM0-101	Add 3 stops for each frame, starting by the one of CDSns	plasmid
A20080	pCM0-104	C-terminal NanoLuc	plasmid
A20081	pCM0-105	Add a Clover (with RBCS2 intron 2) + StrepII tag in C-terminal	plasmid
A20082	pCM0-106	Add an mCerulean3 (with RBCS2 intron 2) + StrepII tag in C-terminal	plasmid
A20083	pCM0-107	Add a gLuc (with RBCS2 intron 2) + StrepII tag in C-terminal	plasmid
A20084	pCM0-108	red FP (RFP) with the intron 1 of RBCS2	plasmid
A20085	pCM0-109	Allows the targetting to the nucleus	plasmid
A20086	pCM0-110	Allows the targetting to the microbodies	plasmid
A20087	pCM0-111	Allows retention in Endoplasmic Reticulum	plasmid
A20088	pCM0-112	Add an mRuby2 (with RBCS2 intron 2) + StrepII tag in C-terminal	plasmid
A20089	pCM0-113	Add an mVenus (with RBCS2 intron 2) + StrepII tag in C-terminal	plasmid
A20090	pCM0-114	3'UTR and Terminator for expression	plasmid
A20091	pCM0-115	3'UTR and Terminator for expression	plasmid
A20092	pCM0-116	3'UTR and Terminator for expression	plasmid
A20093	pCM0-117	3'UTR and Terminator for expression	plasmid
A20094	pCM0-118	3'UTR and Terminator for strong expression	plasmid
A20095	pCM0-119	3'UTR (with intron) and Terminator for expression	plasmid