Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia

Work Hours
Monday to Friday: 830AM - 530PM



Purpose / Description: Erk activity reporter
Reference: A Versatile Toolkit to Produce Sensitive FRET Biosensors to Visualize Signaling in Time and Space.
Verification: None
Depositor(s): Olivier Pertz
Catalog ID: 40012

Product details

Vector backbone: pTriEx4
Vector type: Mammalian Expression, Bacterial Expression, Insect Expression
Backbone size without insert: 4900 bp
Total vector size: 6900 bp
Backbone Manufacturer: Novagen
Copy number: High
Growth strain: Top10
Growth temperature: 37°C
Antibiotics Resistance: Ampicillin
Depositor comment: A genetically encoded fluorescent sensor of ERK activity. 28. PNAS 25(49)
Gene / Insert: Erk activity reporter
Insert size: 1994 bp
Species: Synthetic
Cloning method: Restriction Enzyme
5′ cloning site: PacI (not destroyed)
3′ cloning site: XhoI (not destroyed)
Promoter: CMV
Tag / fusion protein: His-Myc (N terminal on insert)
5′ sequencing primer: ctggttattgtgctgtctc
3′ sequencing primer: tcagtggtatttgtga
Resource information:

A portion of this plasmid was derived from a plasmid made by: Karel Svoboda, Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn