Address
Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia
Work Hours
Monday to Friday: 830AM - 530PM
pCrU6.4-SaCas9 cloning / aphVIII
RM150.00
Purpose / Description: Cloning vector for Staphylococcus aureus Cas9 (SaCas9) guide RNA transcription, controlled by the U6 snRNA promoter #4 (3’ of Cre15.g640800)
Reference: Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9
Catalog ID: CC-pPH339
Product details
Vector type: Synthetic Biology, CRISPR
Copy number: Unknown
Growth strain: XL1-Blue
Growth temperature: 37°C
Gene / Insert: Cas9
Species: Streptococcus aureus
Promoter: U6 snRNA promoter #4 (3’ of Cre15.g640800)
Resource information:
Cloning vector for *Staphylococcus aureus* Cas9 (SaCas9) guide RNA transcription, controlled by the U6 snRNA promoter #4 (3’ of Cre15.g640800). The 2 bp target site can be inserted in a cut-ligation reaction as annealed oligos into an Esp3I cloning site following the protocol of Ann Ran (Ran et al. 213). The immediate 4-bp sequence upstream of the transcriptional start site was changed to ACTT in all U6 constructs to simplify the cloning procedures.
Vector contains an aphVIII cassette for selection on paromomycin.
Selection in * C. reinhardtii*: paromomycin
