Address
Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia
Work Hours
Monday to Friday: 830AM - 530PM
pCrU6.3-SpCas9 cloning
RM150.00
Purpose / Description: Cloning vector for Streptococcus pyogenes Cas9 (SpCas9) single guide RNA transcription, controlled by the U6 snRNA promoter #3 (3′ of Cre8.g371751)
Reference: Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9
Catalog ID: CC-pPH159
Product details
Vector type: Synthetic Biology, CRISPR
Copy number: Unknown
Growth strain: DH10b
Growth temperature: 37°C
Gene / Insert: Cas9
Species: Streptococcus pyogenes
Promoter: U6 snRNA promoter #3 (3′ of Cre8.g371751)
Resource information:
Cloning vector for *Streptococcus pyogenes* Cas9 (SpCas9) single guide RNA transcription, controlled by the U6 snRNA promoter #3 (3′ of Cre8.g371751). The 2 bp target site can be inserted in a cut-ligation reaction as annealed oligos into an Esp3I cloning site following the protocol of Ann Ran (Ran et al. 213). The immediate 4-bp sequence upstream of the transcriptional start site was changed to ACTT in all U6 constructs to simplify the cloning procedures.
