Promoter: U6 snRNA promoter #3 (3′ of Cre8.g371751)
Resource information:
Cloning vector for *Staphylococcus aureus* Cas9 (SaCas9) guide RNA transcription, controlled by the U6 snRNA promoter #3 (3′ of Cre8.g371751). The 2 bp target site can be inserted in a cut-ligation reaction as annealed oligos into an Esp3I cloning site following the protocol of Ann Ran (Ran et al. 213). The immediate 4-bp sequence upstream of the transcriptional start site was changed to ACTT in all U6 constructs to simplify the cloning procedures.
HindIII and KpnI restriction sites can be used for *C. reinhardtii* antibiotic resistance gene insertion (aphVII, aphVIII, ble).