Address
Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia
Work Hours
Monday to Friday: 830AM - 530PM
pCrU6.1-SaCas9 cloning
RM150.00
Purpose / Description: Cloning vector for Staphylococcus aureus Cas9 (SaCas9) guide RNA transcription, controlled by the U6 snRNA promoter #1 (5′ of Cre8.g377251).
Reference: Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9
Catalog ID: CC-pPH193
Product details
Vector type: Synthetic Biology, CRISPR
Copy number: Unknown
Growth strain: DH10b
Growth temperature: 37°C
Gene / Insert: Cas9
Species: Streptococcus aureus
Selectable marker: Ampicilin
Promoter: U6 snRNA promoter #1 (5′ of Cre8.g377251)
Resource information:
Cloning vector for *Staphylococcus aureus* Cas9 (SaCas9) guide RNA transcription, controlled by the U6 snRNA promoter #1 (5′ of Cre8.g377251). The 2 bp target site can be inserted in a cut-ligation reaction as annealed oligos into an Esp3I cloning site following the protocol of Ann Ran (Ran et al. 213). The immediate 4-bp sequence upstream of the transcriptional start site was changed to ACTT in all U6 constructs to simplify the cloning procedures.
HindIII and KpnI restriction sites can be used for *C. reinhardtii* antibiotic resistance gene insertion (aphVII, aphVIII, ble).
