Address
Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia
Work Hours
Monday to Friday: 830AM - 530PM
Multiple Lentiviral Expression System Kit
The Multiple Lentiviral Expression (MuLE) is a modular and flexible system that can be used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.
Publication
A versatile modular vector system for rapid combinatorial mammalian genetics. Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, Catalano A, Busenhart P, Gonçalves AF, Brandt S, Bode PK, Bode-Lesniewska B, Wild PJ, Frew IJ. J Clin Invest. 2015 Apr 1;125(4):1603-19
Description
The MuLE (Multiple Lentiviral Expression) system facilitates the simultaneous introduction of multiple genetic alterations into mammalian cells. The MuLE system kit consists of 91 building block Entry vectors and 5 lentiviral Destination vectors to allow simple and rapid MultiSite Gateway recombination-based cloning of complex polycistronic lentiviruses containing up to five different genetic expression elements. These vectors can induce combinatorial constitutive or inducible gene overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies. The ability to simultaneously alter multiple genes via infection with a single ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput genetic modulation studies in mammalian cells and tissues.
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