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Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia
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MoClo Plant Parts II and Infrastructure Kit
The MoClo Plant Parts II and Infrastructure Kit contains 95 DNA modules compatible with the Modular Cloning (MoClo) system for hierarchical assembly of plant multigene constructs.
Publication
Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system. Gantner J., Ordon J., Ilse T., Kretschmer C., Gruetzner R., Lofke C., Dagdas Y., Burstenbinder K., Marillonnet S. and Stuttmann J. PLoS ONE (2018), 13(5), e0197185. May 30
Description
The MoClo Plant Parts II and Infrastructure Kit contains 95 DNA modules compatible with the Modular Cloning (MoClo) system for hierarchical assembly of plant multigene constructs. Most modules are fully or partially compatible with the GoldenBraid standard.
This kit contains approximately 80 Level 0 modules (phytobricks; e.g. promoters, epitope tags, subcellular localization signals, genes). A further set of Level 0 modules is intended for the assembly of Gateway destination vectors by MoClo. A set of shuttle vectors allows the conversion of MoClo modules containing coding sequences into Gateway entry vectors. Furthermore, the kit contains eight vectors for yeast two hybrid assays or type III secretion system-dependent bacterium-to-plant delivery of proteins compatible with the MoClo system (vectors receive CDS1 or CDS1ns modules). The kit also contains a Golden Gate cloning-compatible TRV-RNA2 vector for virus-induced gene silencing.
Please review Supplemental Tables S2 and S3 from the original publication for more details on using plasmids included in this kit.
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